villabliss.blogg.se

Afterwards, a top layer of Mueller Hinton agar inoculated with 0.2% microbial suspension was poured over the Petri dishes. At twenty–four hours culture of selected bacteria/yeasts was mixed with physiological saline solution and the turbidity was corrected by adding sterile physiological saline until a MacFarland turbidity standard of 0.5 (10 8 CFU/mL). Petri dishes were prepared with a base layer of Mueller Hinton agar. The agar disc diffusion method (12) was used to evaluate the antimicrobial activity by measuring the inhibition zone against the test microorganisms. Two yeasts ( Candida albicans ATCC 10231, Candida krusei ATCC 6258) and seven bacteria ( Enterococcus faecalis ATCC 19433, Escherichia coli ATCC 14948, Kocuria rhizophila ATCC 9341, Neisseria gonorrhoeae ATCC 49226, Pseudomonas aeruginosa ATCC 27853, Shigella flexneri ATCC 12022, Staphylococcus aureus ATCC 25923) and three multi–resistant bacterial strains ( Klebsiella pneumoniae and Pseudomonas aeruginosa isolated from uroculture, and Staphylococcus aureus isolated from hemoculture, resistant to commonly used antimicrobials, as well as sensible to Aztreonam, polymyxin B and vancomycin, respectively) were used to evaluate the antimicrobial activity of Syzygium cumini leaves hydroalcoholic extract. The extract was filtered and concentrated under reduced pressure to obtain the crude hydroalcoholic extract. Powdered air–dried leaves were exhaustively extracted with aqueous 96% ethanol at room temperature by maceration for 30 days. The authenticated plant material was provided by Bom Chá Comercial LTDA, São Paulo, SP, Brazil. The folkloric use of this species to treat infectious diseases stimulated the investigation of the antimicrobial activity of the hydroalcoholic extract from Syzygium cumini leaves against standard and multi–drug resistant Gram–positive and Gram negative human pathogenic bacteria, as well as against yeasts. The leaves are also used to strengthen the teeth and gums, to treat leucorrhoea, stomachalgia, fever, gastropathy, strangury, dermopathy (21), constipation, and to inhibit blood discharges in the faeces (2). Different parts of this plant, such as seeds, bark, fruit, and leaves have been used in traditional medicine as a remedy for diabetes mellitus in many countries (14,18). Syzygium cumini (L.) Skeels (synonym Eugenia jambolana), popularly known as "jambolão", belonging to Myrtaceae family is one of the most commonly medicinal plants used to treat diabetes mellitus in Brazil (17). Palavras–chave: Atividade antimicrobiana, método de difusão, método de microdiluição, cepas multiresistentes, Syzygium cumini O extrato hidroalcoólico mostrou atividade contra Candida krusei (halo de inibição de 14.7 ± 0.3 mm e CIM = 70 µg/mL) e cepas multiresistentes de Pseudomonas aeruginosa, Klebsiella pneumoniae e Staphylococcus aureus. Key–words: Antimicrobial activity, disk diffusion method, microdilution broth method, multi–resistant strains, Syzygium cuminiĪ atividade antimicrobiana do extrato das folhas de Syzygium cumini (Myrtaceae), conhecido como "jambolão", foi avaliada. The crude hydroalcoholic extract was active against Candida krusei (inhibition zone of 14.7 ± 0.3 mm and MIC = 70 µg/mL), and against multi–resistant strains of Pseudomonas aeruginosa, Klebsiella pneumoniae and Staphylococcus aureus. The antimicrobial activity of Syzygium cumini leaves extract, known as "jambolão", was evaluated. IIFaculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, SP, Brasil de Pesquisa em Ciências Exatas e Tecnológicas, Universidade de Franca, Franca, SP, Brasil Armando de Sales Oliveira, 201 – 14404≦00 – Franca – SP – Brasil.

Mailing address: Universidade de Franca – Núcleo de Pesquisa em Ciências Exatas e Tecnológicas – Avenida Dr. Guilherme Ferreira de Oliveira I Niege Araçari Jacometti Cardoso Furtado I Ademar Alves da Silva Filho I Carlos Henrique Gomes Martins I Jairo Kenupp Bastos II Wilson Roberto Cunha I Márcio Luís de Andrade e SilvaI, * *Ĭorresponding Author.

Antimicrobial activity of Syzygium cumini (Myrtaceae) leaves extractĪtividade antimicrobiana do extrato das folhas de Syzygium cumini (Myrtaceae)